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• The intrinsic fluorescence of AuNCls can be directly used for both in vitro and in vivo imaging studies.
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Using a dark field microscope with a high NA lens, light scattered from AuNPs can be imaged as bright spots even though the dimensions of AuNPs are smaller than the diffraction limit of the lens. A major advantage of DFCI is that it allows high-contrast, true-color and multiplexed detection of gold nanoparticles with different sizes and shapes. PA imaging takes advantage of the high resolution of optical imaging and the deeper tissue penetration of ultrasound imaging.

Recently You et al.51 fabricated a visual colorimetric nitrocellulose membrane strip-based sensing assay for Gram positive bacteria by using vancomycin-modified gold nanoparticles and one-pot reaction. They took advantage of the broad spectrum inhibiting activity against Gram-positive bacteria of the vancomycin antibiotic and 9.5 nm spherical AuNPs. The assay could differentiate between Gram-negative and Gram-positive bacteria. The obtained detection limits with the naked eye for all Gram-positive S. Subtilis were 1 × 109 cells per mL, and there was no interaction of the synthesized complex with Gram-negative bacteria such as E. DFCI can detect AuNPs that are as small as 10 nm in diameter.

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The zeta potential value of the nanohybrid ratio was 7.7–38.4 mV. Aureus confirming its application in label-free and rapid bio-sensing of microorganisms and target biomolecules. Under optimal conditions, a low limit of detection of 4 CFU per mL was obtained for the detection of S.

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